Hints & Tips

Hanson's Helpful Hints (or handy things to know)

  • By using the small (ca. 1/2 inch OD) vacuum tubing and a plastic syringe, you can pipette cleanly and with fairly accurate delivery volumes. Stretch one end of a one inch length of tubing over a 2 ml plastic syringe, and insert your pipettes into the other end of the hose. Much longer lived than standard pipette bulbs and less messy.
  • Use a quick connect on an air hose (the small end of the quick connect) to push solvents through microscale columns made from pipettes. It's much more convenient than pipette bulbs.
  • When combining column fractions, use "vacuum adapters" common for distillations . It's the piece that has a female joint on top, a mail joint on bottom and a side-arm. Equip the top with a rubber septum and teflon cannula to combine column fractions. Apply a vacuum using an aspirator and siphon your fractions into a flask.
  • Use a long needle attached to an air hose to dry your NMR tubes.
  • The 200 MW rule... anything under 200 molecular weight won't survive long on a high vacuum system.
  • Use a 16x100 test tube and 14/20 yellow "caplug" for small scale extractions. The "caplug" fits tightly enough that the tube can be inverted without spillage.
  • The empty automatic pipetter tip cases (for 200 microliter) make excellent stands for NMR tubes.
  • When using microliter syringes with metal complexes, such as Titanium Isopropoxide, rinse it immediately afterward withorganic solvent followed by acid, then clean as usual.
  • Never ever use a brush to clean out a high pressure reaction vessel or pressure tube. The brush can make tiny scratches on the inside and cause the glass to fail at high pressure.
  • TLC stain recipes

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